| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gregory, Elizabeth F (10)
|
VAWAC |
32.77 |
32.90 |
0.13 |
| 2 |
Fary, Brenna E (10)
|
VAECAT |
36.17 |
34.61 |
-1.56 |
| 3 |
Rodrigues, Gabriella A (10)
|
VASEVA |
38.51 |
37.84 |
-0.67 |
| 4 |
Campbell, Amelia D (10)
|
VASEVA |
37.35 |
37.96 |
0.61 |
| 5 |
Stupar, Aliyah G (10)
|
VASEVA |
36.64 |
38.77 |
2.13 |
| 6 |
Aquino, Meagan E (10)
|
VAECAT |
43.85 |
39.61 |
-4.24 |
| 7 |
Graves, Bethany H (10)
|
VASEVA |
|
39.67 |
- |
| 8 |
McGowan, Elly G (10)
|
VASEVA |
38.96 |
40.94 |
1.98 |
| 9 |
Shaltayeva, Victoria (10)
|
VASEVA |
39.84 |
41.26 |
1.42 |
| 10 |
Jimenez, Saniya N (10)
|
VASEVA |
37.41 |
41.42 |
4.01 |
| 11 |
Townsend, Leah C (10)
|
VAWAC |
41.68 |
41.95 |
0.27 |
| 12 |
Phillips, Chloe E (10)
|
VAWAC |
42.20 |
42.14 |
-0.06 |
| 13 |
Landon, Emma M (10)
|
VAECAT |
|
42.23 |
- |
| 14 |
Banks, Hannah M (10)
|
VASEVA |
41.56 |
42.54 |
0.98 |
| 15 |
Kohler, Olivia A (10)
|
VATAC |
43.71 |
44.14 |
0.43 |
| 16 |
Wojcik, Melissa A (10)
|
VASEVA |
42.28 |
45.29 |
3.01 |